Flag label purification protein resin features and advantages - Database & Sql Blog Articles

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Due to its hydrophilic nature, the Flag tag is often located on the surface of the fusion protein and is therefore relatively easy to be accessed and recognized by the antibody. Different Flag tag antibodies have different recognition and binding properties to the Flag tag. Abbkine provides a monoclonal antibody with clone number 1B10, which is one of the most specific and potent Flag antibodies on the market.

Product Name Product Form Item Specification

PurKine Anti-DDDDK Tag Resin 4FF Agarose Resin

BMR21504

PurKine Anti-Flag resin (4% cross-linking) is a versatile affinity product for the purification of fusion proteins containing FLAG-tag, which can be reused without regeneration, but with the increase of non-specifically bound proteins and protein aggregation It often causes the flow rate and the combined load to decrease, and the resin can be cleaned and the performance can be restored. Samples are recommended to be centrifuged or filtered through a 0.22μm or 0.45μm filter prior to loading to reduce impurities, improve protein purification efficiency and prevent plugging of the column. The product is based on a highly crosslinked 4% agarose gel and withstands pressures up to 0.3 MPa. It can be combined with peristaltic pumps and AKTA instruments for industrial purification.

Features and advantages

High capacity - more than 1 mg of DYKDDDDK labeled protein per ml of resin.

Cost-effective - performance is degraded after repeated use of the same batch of resin for at least five times.

Flexible - multiple formats including bulk resin, spin columns and kits.

The sturdy-highly crosslinked beads can withstand linear flow rates up to 300 cm/hr.

Figure 1. Abbkine PurKine is a unique combination of purification products consisting of settled resin, pre-packed spin columns and complete kits for the purification and preparation of most samples.

Figure 2. Compared to traditional resin production methods, Abbkine is using the latest synthesis processes, using cutting-edge technology for resin development and component optimization. PurKine tools can be used to transform your samples from fusion proteins, nucleic acids, antibodies and biomolecules to increase productivity, streamline workflow and cost savings.

Abbkine is committed to providing the latest products and technologies for life science research and medical development worldwide. Abbkine designs, develops, manufactures and markets a unique range of products, including antibodies, research reagents, and cell functional analysis products and protein purification products for life science research. Their products provide effective solutions to the challenges of scientific research, saving time and increasing research efficiency for scientists around the world. As the general agent of Abbkine in China, Aijiejie Technology Co., Ltd., with its rich professional experience, will continue to provide Abbkine quality products and technical services to customers in China.