High-end UV-visible spectrophotometry application - Database & Sql Blog Articles

Application of high-end UV-visible spectrophotometry

Since the advent of UV-visible spectrophotometry, there has been great development in application, especially on the basis of the development of related disciplines, which has promoted continuous innovation of spectrophotometer instruments, enabling automatic recording, automatic printing, digital display, and microcomputer Control and other technologies have made the sensitivity and accuracy of photometry more and more, and its application range has been expanding.

The Lambda 1050î“”950 series UV-visible spectrophotometer developed by PerkinElmer of the United States and the Agilent Cary6000i UV-Vis NIR spectrophotometer represent the world's leading level of such instruments. Beijing Pu's TU-1950 series UV-visible spectrophotometer and Meifang (China) Instrument Co., Ltd. UV-1900 UV-visible spectrophotometer have higher technical level in the domestic market, but there is still a certain gap with similar foreign products.

The application of UV-visible spectrophotometer is very extensive. Here, the small series of China Measurement Tool Network introduces the related applications of UV-Vis spectrophotometer in the industry.

For water and wastewater monitoring applications, monitoring and comprehensive evaluation of a water system generally includes the aqueous phase (solution itself), solid phase (suspended matter, sediment), and biological phase (aquatic organism). In the routine monitoring of water quality, UV-visible spectrophotometry has a large proportion. Since the composition of water and wastewater is complex and variable, the concentration of the analyte and the concentration of the interferent are very different, and the analysis method must be selected in the specific analysis.

The components or ingredients that can be used for detection in agricultural product and food analysis are protein, lysine, glucose, vitamin C, nitrate, nitrite, arsenic, mercury, and the like;

It can be used to detect the activity of chlorophyll, total nitrogen and enzymes in plant biochemical analysis;

It can be used to detect niacin, gossypol, phosphine and methyl ester in feed analysis.

The UV-Vis spectrophotometer reacts with methyl orange in a pH of 5.0 to form a colored complex. The concentration of the complex in the chloroform liquid is proportional to its absorbance at visible wavelengths. The determination of nicotine content in the waste tobacco extract was carried out by spectrophotometer. The chlorophyll content of wheat leaf was measured by spectrophotometer. The results showed that the chlorophyll content of wheat leaves in both dry and fresh conditions was not affected without losing green.

Ultraviolet-visible spectrophotometry has a wide range of instruments and low applicability. Especially after the computer control, the technology has developed rapidly and its functions will be more complete, making the photometric method continue to broaden the application range and become the best for analysts. select. We will also expect UV-Vis spectrophotometry to be further developed and applied in more industry sectors.